Paul Wiseman
Professor
Development and cellular applications of imaging based fluorescence fluctuation methods including image correlation spectroscopy (ICS), single-molecule step photobleaching analysis, and spatial intensity distribution analysis (SpIDA) to measure receptor transport, densities, and oligomerization state within intact cells and neurons.
Characterization of cellular signalling, receptor聽 and protein transport and oligomerization and hetero-protein interactions in living cells and neurons with application to cell migration, signal transduction, axon pathfinding and synaptic formation. The biophysical image analysis toolsets enhance our microscopy development providing a way to measure protein interactions and macromolecular transport properties for cell adhesion macromolecules (in focal adhesions & podosomes, integrin cytoskeletal interactions); neuron pathfinding (growth cones, netrin-1 guidance cue, netrin receptors); cell signalling (EGF receptor clustering; GPCR oligomerization and transactivation; CFTR channel organization, clustering and regulation in the membrane).
Application of new nonlinear microscopic techniques such as second and third harmonic generation (SHG/THG) microscopy for imaging collagen extracellular matrices, metallic nanoparticles and for and for detecting malaria hemozoin within infected blood cells.
Current and future research is extending the fluorescence fluctuation methods to new imaging modalities including 3D in time with light/lattice sheet microscopy and to super-resolution microscopy with SIM, PALM, SOFI and STED imaging.