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Zeiss LSM710 - Airyscan

Zeiss LSM710 Airyscan
Image by Emily Pruner - 2020.

EQUIPMENT FEATURES SPECIFICATIONS

Stand model

AxioObserver Z1 (Inverted)

Features and Applications

Laser scanning confocal microscope

Brightfield, Phase, DIC

4D Imaging (XY, Z-stack, Time-lapse)

Multi-Channel

FRET

FRAP

Spectral imaging.

Live cell imaging.

Tiling.

Multipoint

Definite Focus

Environmental chamber (CO2, O2, humidity and temperature control)

Inserts: Slides, culture cell plates and dishes

Light Sources

Laser lines:

405 nm (Diode, 30mW)

458 nm, 488 nm, 514 nm (Argon, 25mW)

543 nm (HeNe, 1mW)

594 nm (HeNe, 2mW)

633 nm (HeNe, 5mW)

Transmitted Halogen light

X-Cite 120Q

Objectives

5x, EC EpiPlan Neofluar, DIC, NA = 0.13

20x Plan Apochromat, DIC, NA = 0.8

40x/ Plan Apochromat, DIC, NA = 1.4, Oil

63x Plan Apochromat, DIC, NA = 1.4, Oil

63x C-Apochromat, DIC, NA = 1.2, Water, Corr 0.14-.019

100x/ Plan Apochromat, DIC, NA= 1.4 Oil

Filter sets

: BP 365/12, FT 395, LP 397 (DAPI, BFP)

: BP 470/20, FT 510, BP 538/50 (Alexa 488, CFDA, FM1-43)

: G 365, FT 395, BP445/50 (DAPI, Hoechst)

Analyzer module Pol ACR P&C for transmitted light

Software

Zen

Detectors

Transmitted PMT (T-PMT)

Gallium arsenide phosphide (GaAsP) PMT

Airyscan

Location

P-027

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